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KMID : 0545119980080050441
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 5 p.441 ~ p.448
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Development of Cellobiose-utilizing Recombinant Yeast for Ethanol Production from Cellulose Hydrolyzate
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Pack, Seung Pil
Cho, Kwang Myung/Kang, Hyen Sam/Yoo, Young Je
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Abstract
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A cellobiose-utilizing recombinant yeast having β-glucosidase activity was developed for ethanol production from a mixture of glucose and cellobiose. Using δ-sequences of Ty1 transposon of yeast as target sites for homologous recombination, a heterologous gene of β-glucosidase was integrated into the chromosome of Saccharomyces cerevisiae. The δ-integrated recombinant yeast, Saccharomyces cerevisiae L2612 (δ-BGL), showed perfect mitotic stability even in nonselective media and showed ca. 1.5 fold higher β-glucosidase activity than the recombinant yeast harboring the 2μ-based plasmid vector system. A mathematical model was developed to describe the β-glucosidase formation and ethanol production from the Saccharomyces cerevisiae L2612 (pδ-BGL). The model newly described that the heterologous (3-glucosidase production mediated by ADH1 promoter is regulated by glucose and repressed by ethanol.
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KEYWORD
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